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首页> 外文OA文献 >Optical Coherence Microscopy. A Technology for Rapid, in Vivo, Non-Destructive Visualization of Plants and Plant Cells
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Optical Coherence Microscopy. A Technology for Rapid, in Vivo, Non-Destructive Visualization of Plants and Plant Cells

机译：光学相干显微镜。快速，无损可视化植物和植物细胞的技术

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We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events in shoot tissues without causing damage. With OCM we can image cells or groups of cells that are up to 1 mm deep in living tissues, resolving structures less than 5 μm in size, with a typical collection time of 5 to 6 min. OCM measures the inherent light-scattering properties of biological tissues and cells. These optical properties vary and provide endogenous developmental markers. Singly scattered photons from small (e.g. 5x3x5x3x10 μm) volume elements (voxels) are collected, assembled, and quantitatively false-colored to form a threedimensional image. These images can be cropped or sliced in any plane. Adjusting the colors and opacities assigned to voxels allows us to enhance different features within the tissues and cells. We show that light-scattering properties are the greatest in regions of the Arabidopsis shoot undergoing developmental processes. In large cells, high light scattering is produced from nuclei, intermediate light scatter is produced from cytoplasm, and little if any light scattering originates from the vacuole and cell wall. OCM allows the rapid, repetitive, non-destructive collection of quantitative data about inherent properties of cells, so it provides a means of continuously monitoring plants and plant cells during development and in response to exogenous stimuli.
机译：我们描述了一种用于植物生物学的新成像技术，光学相干显微镜（OCM）的开发和利用，该技术可以对植物和植物细胞进行真正的体内可视化。这项新颖的技术可以直接在原地（例如土壤中的植物）对芽组织中的细胞和事件进行三维可视化，而不会造成损坏。借助OCM，我们可以对活组织中最深1毫米的细胞或细胞组成像，分辨尺寸小于5μm的结构，典型采集时间为5至6分钟。 OCM测量生物组织和细胞的固有光散射特性。这些光学性质变化并提供内源性发育标记。来自小（例如5x3x5x3x10μm）体积元素（体素）的单个散射光子被收集，组装并定量地伪彩色以形成三维图像。这些图像可以在任何平面上裁剪或切片。调整分配给体素的颜色和不透明度可以使我们增强组织和细胞内的不同特征。我们显示光散射特性是拟南芥芽区域经历发展过程中最大的。在大细胞中，高光散射是由细胞核产生的，中间光散射是由细胞质产生的，几乎没有任何光散射来自液泡和细胞壁。 OCM允许快速，重复，非破坏性地收集有关细胞固有特性的定量数据，因此它提供了一种在发育过程中以及对外源刺激作出反应时连续监测植物和植物细胞的方法。

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